SPARC Mouse Monoclonal Antibody [Clone ID: OTI2B1]

CAT#: CF507359

Carrier-free (glycerol/BSA-free) SPARC mouse monoclonal antibody, clone OTI2B1 (formerly 2B1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPARC ([TA507361], Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPARC. Positive lysates LY418873 (100ug) and LC418873 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-SPARC mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA507359)

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SPARC mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA507359)

Specifications

Product Data

• Clone Name: OTI2B1
• Applications: IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SPARC(NP_003109) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34.5 kDa
• Gene Name: Homo sapiens secreted protein acidic and cysteine rich (SPARC), transcript variant 1, mRNA.
• Database Link:
  NP_003109
  Entrez Gene 20692 MouseEntrez Gene 24791 RatEntrez Gene 6678 Human
  UniProt ID P09486
• Background: This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. [provided by RefSeq, Dec 2011]
• Synonyms: BM-40; OI17; ON

Reference Data

• Protein Families: Druggable Genome