SPARC Mouse Monoclonal Antibody [Clone ID: OTI2B1]

CAT#: CF507359

Carrier-free (glycerol/BSA-free) SPARC mouse monoclonal antibody, clone OTI2B1 (formerly 2B1)

Formulation: Standard Carrier Free


  View other "OTI2B1" antibodies (2)

USD 465.00

In Stock*

Size
    • 100 ug

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Specifications

Product Data
Clone Name OTI2B1
Applications IHC, WB
Recommended Dilution WB 1:4000, IHC 1:150
Reactivities Human, Mouse, Rat
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Full length human recombinant protein of human SPARC(NP_003109) produced in HEK293T cell.
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 34.5 kDa
Gene Name Homo sapiens secreted protein acidic and cysteine rich (SPARC), transcript variant 1, mRNA.
Background This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. [provided by RefSeq, Dec 2011]
Synonyms BM-40; OI17; ON
Reference Data
Protein Families Druggable Genome

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