UBE2E3 Mouse Monoclonal Antibody [Clone ID: OTI6D10]
CAT#: CF800072
Carrier-free (BSA/glycerol-free) UBE2E3 mouse monoclonal antibody, clone OTI6D10 (formerly 6D10)
Formulation: Standard
Other products for "UBE2E3"
Specifications
Product Data | |
Clone Name | OTI6D10 |
Applications | WB |
Recommended Dilution | WB 1:500~2000 |
Reactivities | Human, Monkey, Mouse, Rat |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Full length human recombinant protein of human UBE2E3 (NP_006348) produced in E.coli. |
Formulation | Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose) |
Reconstitution Method | For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific) |
Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
Conjugation | Unconjugated |
Storage | Store at -20°C as received. |
Stability | Stable for 12 months from date of receipt. |
Predicted Protein Size | 22.7 kDa |
Gene Name | Homo sapiens ubiquitin conjugating enzyme E2 E3 (UBE2E3), transcript variant 1, mRNA. |
Database Link | |
Background | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
Synonyms | UBCH9; UbcM2 |
Reference Data | |
Protein Pathways | Ubiquitin mediated proteolysis |
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