TRIM9 Mouse Monoclonal Antibody [Clone ID: OTI1E4]

CAT#: CF800073

Carrier-free (BSA/glycerol-free) TRIM9 mouse monoclonal antibody, clone OTI1E4 (formerly 1E4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM9 (RC201423, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM9. Positive lysates LY409342 (100ug) and LC409342 (20ug) can be purchased separately from OriGene.

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  Anti-TRIM9 mouse monoclonal antibody (TA800073) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TRIM9 (RC201423).

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  HEK293T cells transfected with either RC201423 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TRIM9 antibody (TA800073), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1E4
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 284-669 of human TRIM9 (NP_443210) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 61.2 kDa
• Gene Name: Homo sapiens tripartite motif containing 9 (TRIM9), transcript variant 2, mRNA.
• Database Link:
  NP_443210
  Entrez Gene 114088 Human
  UniProt ID Q9C026
• Background: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Alternate splicing of this gene generates two transcript variants encoding different isoforms. [provided by RefSeq]
• Synonyms: RNF91; SPRING

Reference Data

• Protein Families: Druggable Genome