TMS1 (PYCARD) Mouse Monoclonal Antibody [Clone ID: OTI1A2]

CAT#: CF800079

Carrier-free (BSA/glycerol-free) PYCARD mouse monoclonal antibody, clone OTI1A2 (formerly 1A2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PYCARD (RC215592, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCARD. Positive lysates LY402233 (100ug) and LC402233 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PYCARD monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  HEK293T cells transfected with either RC215592 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PYCARD antibody (TA800079), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1A2
• Applications: FC, WB
• Recommended Dilution: WB 1:500~2000, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PYCARD (NP_037390) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 21.4 kDa
• Gene Name: Homo sapiens PYD and CARD domain containing (PYCARD), transcript variant 1, mRNA.
• Database Link:
  NP_037390
  Entrez Gene 29108 Human
  UniProt ID Q9ULZ3
• Background: This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: ASC; CARD5; TMS; TMS-1; TMS1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cytosolic DNA-sensing pathway, NOD-like receptor signaling pathway