C1R Mouse Monoclonal Antibody [Clone ID: OTI1F1]

CAT#: CF800085

Carrier-free (BSA/glycerol-free) C1R mouse monoclonal antibody, clone OTI1F1 (formerly 1F1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C1R (RC223007, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C1R. Positive lysates LY400654 (100ug) and LC400654 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-C1R monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Specifications

Product Data

• Clone Name: OTI1F1
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Dog
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 262-562 of human C1R (NP_001724) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 80 kDa
• Gene Name: Homo sapiens complement C1r (C1R), transcript variant 1, mRNA.
• Database Link:
  NP_001724
  Entrez Gene 477707 DogEntrez Gene 715 Human
  UniProt ID P00736
• Synonyms: complement component 1; r subcomponent

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Complement and coagulation cascades, Systemic lupus erythematosus