Proteasome subunit alpha type 6 (PSMA6) Mouse Monoclonal Antibody [Clone ID: OTI7C2]

CAT#: CF800122

Carrier-free (BSA/glycerol-free) PSMA6 mouse monoclonal antibody, clone OTI7C2 (formerly 7C2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMA6 (RC209976, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMA6. Positive lysates LY419119 (100ug) and LC419119 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PSMA6 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800122)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800122)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800122)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800122)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800122)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800122)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800122)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800122)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800122)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800122)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800122)

• HEK293T cells transfected with either RC209976 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PSMA6 antibody (TA800122), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI7C2
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PSMA6 (NP_002782) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 27.2 kDa
• Gene Name: Homo sapiens proteasome subunit alpha 6 (PSMA6), transcript variant 1, mRNA.
• Database Link:
  NP_002782
  Entrez Gene 26443 MouseEntrez Gene 29673 RatEntrez Gene 480290 DogEntrez Gene 695113 MonkeyEntrez Gene 5687 Human
  UniProt ID P60900
• Background: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. A pseudogene has been identified on the Y chromosome. [provided by RefSeq]
• Synonyms: IOTA; p27K; PROS27

Reference Data

• Protein Families: Druggable Genome, Protease, Stem cell - Pluripotency
• Protein Pathways: Proteasome