SUHW1 (ZNF280A) Mouse Monoclonal Antibody [Clone ID: OTI3G4]

CAT#: CF800212

Carrier-free (BSA/glycerol-free) ZNF280A mouse monoclonal antibody, clone OTI3G4 (formerly 3G4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZNF280A (RC208802, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZNF280A. Positive lysates LY409057 (100ug) and LC409057 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ZNF280A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800212)

Specifications

Product Data

• Clone Name: OTI3G4
• Applications: IHC, WB
• Recommended Dilution: IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-333 of human ZNF280A (NP_542778) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 60.6 kDa
• Gene Name: Homo sapiens zinc finger protein 280A (ZNF280A)
• Database Link:
  NP_542778
  Entrez Gene 129025 Human
  UniProt ID P59817
• Background: This gene was predicted both by automated computational analysis and by similarity to a Drosophila gene and to predicted genes in other species (sheep, chimp, dog, cow). The predicted protein of this gene is similar to Drosophila suppressor of hairy wing protein, a leucine zipper protein which represses the function of transcriptional enhancers of the gypsy retrotransposon. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: 3 OY11.1; 3'OY11.1; SUHW1; ZNF280; ZNF636

Reference Data

• Protein Families: Druggable Genome, Transcription Factors