AGTPBP1 Mouse Monoclonal Antibody [Clone ID: OTI9D11]

CAT#: CF800251

Carrier-free (BSA/glycerol-free) AGTPBP1 mouse monoclonal antibody, clone OTI9D11 (formerly 9D11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AGTPBP1 (RC209238, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AGTPBP1. Positive lysates LY414709 (100ug) and LC414709 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AGTPBP1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Specifications

Product Data

• Clone Name: OTI9D11
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 368-753 of human AGTPBP1 (NP_056054) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens ATP/GTP binding protein 1 (AGTPBP1), transcript variant 2, mRNA.
• Database Link:
  NP_056054
  Entrez Gene 67269 MouseEntrez Gene 290986 RatEntrez Gene 23287 Human
  UniProt ID Q9UPW5
• Background: NNA1 is a zinc carboxypeptidase that contains nuclear localization signals and an ATP/GTP-binding motif that was initially cloned from regenerating spinal cord neurons of the mouse. [supplied by OMIM, Jul 2002]
• Synonyms: CCP1; NNA1

Reference Data

• Protein Families: Protease