Methionine Aminopeptidase 2 (METAP2) Mouse Monoclonal Antibody [Clone ID: OTI1F6]

CAT#: CF800289

Carrier-free (BSA/glycerol-free) METAP2 mouse monoclonal antibody, clone OTI1F6 (formerly 1F6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY METAP2 (RC203809, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-METAP2. Positive lysates LY402045 (100ug) and LC402045 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (10ug) from 6 different cell lines by using anti-METAP2 monoclonal antibody (1:200).

• 

  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-METAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800289)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-METAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800289)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-METAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800289)

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-METAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800289)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-METAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800289)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-METAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800289)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-METAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800289)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-METAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800289)

• Anti-METAP2 mouse monoclonal antibody (TA800289) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY METAP2 (RC203809).

• HEK293T cells transfected with either RC203809 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-METAP2 antibody (TA800289), and then analyzed by flow cytometry.

Show all 12 images

Specifications

Product Data

• Clone Name: OTI1F6
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 2-212 of human METAP2 (NP_006829) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52.7 kDa
• Gene Name: Homo sapiens methionyl aminopeptidase 2 (METAP2), transcript variant 1, mRNA.
• Database Link:
  NP_006829
  Entrez Gene 56307 MouseEntrez Gene 64370 RatEntrez Gene 10988 Human
  UniProt ID P50579
• Background: This gene is a member of the methionyl aminopeptidase family and encodes a protein that binds 2 cobalt or manganese ions. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. Increased expression of this gene is associated with various forms of cancer and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. A pseudogene of this gene is located on chromosome 2. [provided by RefSeq]
• Synonyms: MAP2; MNPEP; p67eIF2

Reference Data

• Protein Families: Druggable Genome, Protease