TLE 1 (TLE1) Mouse Monoclonal Antibody [Clone ID: OTI2D8]

CAT#: CF800318

Carrier-free (BSA/glycerol-free) TLE1 mouse monoclonal antibody, clone OTI2D8 (formerly 2D8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TLE1 (RC204037, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TLE1. Positive lysates LY417561 (100ug) and LC417561 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800318)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800318)

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  Anti-TLE1 mouse monoclonal antibody (TA800318) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TLE1 (RC204037).

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  HEK293T cells transfected with either RC204037 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TLE1 antibody (TA800318), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI2D8
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 180-460 of human TLE1 (NP_005068) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 83 kDa
• Gene Name: Homo sapiens TLE family member 1, transcriptional corepressor (TLE1), transcript variant 2, mRNA.
• Database Link:
  NP_005068
  Entrez Gene 21885 MouseEntrez Gene 362533 RatEntrez Gene 7088 Human
  UniProt ID Q04724
• Synonyms: ESG; ESG1; GRG1

Reference Data

• Protein Families: Transcription Factors