NEIL1 Mouse Monoclonal Antibody [Clone ID: OTI1D1]

CAT#: CF800344

Carrier-free (BSA/glycerol-free) NEIL1 mouse monoclonal antibody, clone OTI1D1 (formerly 1D1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEIL1 (RC202329, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEIL1. Positive lysates LY411199 (100ug) and LC411199 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1D1
• Applications: WB
• Recommended Dilution: WB 1:200
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 186-390 of human NEIL1 (NP_078884) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43.5 kDa
• Gene Name: Homo sapiens nei like DNA glycosylase 1 (NEIL1), transcript variant 2, mRNA.
• Database Link:
  NP_078884
  Entrez Gene 79661 Human
  UniProt ID Q96FI4
• Background: This gene is a member of the Nei endonuclease VIII-like gene family which encodes DNA glycosylases. The encoded enzyme participates in the DNA repair pathway by initiating base excision repair by removing damaged bases, primarily oxidized pyrimidines. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]
• Synonyms: FPG1; hFPG1; NEI1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair