DPPA2 Mouse Monoclonal Antibody [Clone ID: OTI1G10]

CAT#: CF800361

Carrier-free (BSA/glycerol-free) DPPA2 mouse monoclonal antibody, clone OTI1G10 (formerly 1G10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DPPA2 (RC205441, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DPPA2.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DPPA2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Specifications

Product Data

• Clone Name: OTI1G10
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-175 of human DPPA2 (NP_620170) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.6 kDa
• Gene Name: Homo sapiens developmental pluripotency associated 2 (DPPA2), mRNA.
• Database Link:
  NP_620170
  Entrez Gene 685364 RatEntrez Gene 607166 DogEntrez Gene 151871 Human
  UniProt ID Q7Z7J5
• Synonyms: CT100; ECAT15-2; PESCRG1

Reference Data

• Protein Families: Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency