Isocitrate dehydrogenase (IDH1) Mouse Monoclonal Antibody [Clone ID: OTI3G9]

CAT#: CF800406

Carrier-free (BSA/glycerol-free) IDH1 mouse monoclonal antibody, clone OTI3G9 (formerly 3G9)

Formulation: Standard Carrier Free


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USD 465.00

In Stock*

Size
    • 100 ug

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Specifications

Product Data
Clone Name OTI3G9
Applications IHC, WB
Recommended Dilution WB 1:2000, IHC 1:150
Reactivities Human, Mouse, Rat, Dog
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Synthetic peptide around the R132H mutation region of the human IDH conjugated to KLH
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 46.5 kDa
Gene Name Homo sapiens isocitrate dehydrogenase (NADP(+)) 1, cytosolic (IDH1), transcript variant 1, mRNA.
Background Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq, Jul 2008]
Synonyms HEL-216; HEL-S-26; IDCD; IDH; IDP; IDPC; PICD
Reference Data
Protein Pathways Citrate cycle (TCA cycle), Glutathione metabolism, Metabolic pathways

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