Epoxide hydrolase (EPHX1) Mouse Monoclonal Antibody [Clone ID: OTI3F10]

CAT#: CF800407

Carrier-free (BSA/glycerol-free) EPHX1 mouse monoclonal antibody, clone OTI3F10 (formerly 3F10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPHX1 (RC200621, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPHX1. Positive lysates LY400042 (100ug) and LC400042 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EPHX1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-EPHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800407)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-EPHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800407)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-EPHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800407)

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Specifications

Product Data

• Clone Name: OTI3F10
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 21-230 of human EPHX1 (NP_000111) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52.8 kDa
• Gene Name: Homo sapiens epoxide hydrolase 1 (EPHX1), transcript variant 1, mRNA.
• Database Link:
  NP_000111
  Entrez Gene 13849 MouseEntrez Gene 25315 RatEntrez Gene 700180 MonkeyEntrez Gene 2052 Human
  UniProt ID P07099
• Background: Epoxide hydrolase is a critical biotransformation enzyme that converts epoxides from the degradation of aromatic compounds to trans-dihydrodiols which can be conjugated and excreted from the body. Epoxide hydrolase functions in both the activation and detoxification of epoxides. Mutations in this gene cause preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2008]
• Synonyms: EPHX; EPOX; HYL1; MEH

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Metabolism of xenobiotics by cytochrome P450