Epoxide hydrolase (EPHX1) Mouse Monoclonal Antibody [Clone ID: OTI2C1]

CAT#: CF800408

Carrier-free (BSA/glycerol-free) EPHX1 mouse monoclonal antibody, clone OTI2C1 (formerly 2C1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPHX1 (RC200621, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPHX1. Positive lysates LY400042 (100ug) and LC400042 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC200621 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-EPHX1 antibody (TA800408), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI2C1
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 21-230 of human EPHX1 (NP_000111) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52.8 kDa
• Gene Name: Homo sapiens epoxide hydrolase 1 (EPHX1), transcript variant 1, mRNA.
• Database Link:
  NP_000111
  Entrez Gene 2052 Human
  UniProt ID P07099
• Background: Epoxide hydrolase is a critical biotransformation enzyme that converts epoxides from the degradation of aromatic compounds to trans-dihydrodiols which can be conjugated and excreted from the body. Epoxide hydrolase functions in both the activation and detoxification of epoxides. Mutations in this gene cause preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
• Synonyms: EPHX; EPOX; HYL1; MEH

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Metabolism of xenobiotics by cytochrome P450