ZSCAN4 Mouse Monoclonal Antibody [Clone ID: OTI3B1]

CAT#: CF800448

Carrier-free (BSA/glycerol-free) ZSCAN4 mouse monoclonal antibody, clone OTI3B1 (formerly 3B1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZSCAN4 (RC211003, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZSCAN4. Positive lysates LY407355 (100ug) and LC407355 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ZSCAN4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Specifications

Product Data

• Clone Name: OTI3B1
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Dog
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 164-433 of human ZSCAN4 (NP_689890) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 48.8 kDa
• Gene Name: Homo sapiens zinc finger and SCAN domain containing 4 (ZSCAN4), mRNA.
• Database Link:
  NP_689890
  Entrez Gene 106559239 DogEntrez Gene 201516 Human
  UniProt ID Q8NAM6
• Background: The ZSCAN4 gene encodes a protein involved in telomere maintenance and with a key role in the critical feature of mouse embryonic stem (ES) cells, namely, defying cellular senescence and maintaining normal karyotype for many cell divisions in culture (Zalzman et al., 2010 [PubMed 20336070]). [supplied by OMIM, May
• Synonyms: ZNF494

Reference Data

• Protein Families: Transcription Factors