SUMF1 Mouse Monoclonal Antibody [Clone ID: OTI1B10]

CAT#: CF800632

Carrier-free (BSA/glycerol-free) SUMF1 mouse monoclonal antibody,clone OTI1B10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SUMF1 (RC211092, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SUMF1 (1:500). Positive lysates LY405331 (100ug) and LC405331 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1B10
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SUMF1 (NP_877437) produced in HEK293T cell
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 37.3 kDa
• Gene Name: Homo sapiens sulfatase modifying factor 1 (SUMF1), transcript variant 1, mRNA.
• Database Link:
  NP_877437
  Entrez Gene 58911 MouseEntrez Gene 362409 RatEntrez Gene 285362 Human
  UniProt ID Q8NBK3
• Background: This gene encodes an enzyme that catalyzes the hydrolysis of sulfate esters by oxidizing a cysteine residue in the substrate sulfatase to an active site 3-oxoalanine residue, which is also known as C-alpha-formylglycine. Mutations in this gene cause multiple sulfatase deficiency, a lysosomal storage disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]
• Synonyms: AAPA3037; FGE; UNQ3037