TIA1 Mouse Monoclonal Antibody [Clone ID: OTI2B1]

CAT#: CF800634

Carrier-free (BSA/glycerol-free) TIA1 mouse monoclonal antibody, clone OTI2B1 (formerly 2B1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TIA1 (RC219386, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TIA1. Positive lysates LY411728 (100ug) and LC411728 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2B1
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TIA1 (NP_071505) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens TIA1 cytotoxic granule associated RNA binding protein (TIA1), transcript variant 2, mRNA.
• Database Link:
  NP_071505
  Entrez Gene 21841 MouseEntrez Gene 312510 RatEntrez Gene 7072 Human
  UniProt ID P31483
• Synonyms: TIA-1; WDM

Reference Data

• Protein Families: Druggable Genome