Cytokeratin 5 (KRT5) Mouse Monoclonal Antibody [Clone ID: OTI6F8]

CAT#: CF800727

Carrier-free (BSA/glycerol-free) KRT5 mouse monoclonal antibody, clone OTI6F8 (formerly 6F8)

Product Images

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-KRT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800727)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-KRT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800727)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-KRT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800727)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-KRT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800727)

Specifications

Product Data

• Clone Name: OTI6F8
• Applications: IHC
• Recommended Dilution: IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide: EQGTKTVRQN LEPLFEQYIN NLRRQ, corresponding to the conserved region of human KRT5 and KRT6
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 62.2 kDa
• Gene Name: Homo sapiens keratin 5 (KRT5), mRNA.
• Database Link:
  NP_000415
  Entrez Gene 110308 MouseEntrez Gene 369017 RatEntrez Gene 3852 Human
  UniProt ID P13647
• Background: The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
• Synonyms: CK5; DDD; DDD1; EBS2; K5; KRT5A