CD99 Mouse Monoclonal Antibody [Clone ID: OTI5C1]

CAT#: CF800788

Carrier-free (BSA/glycerol-free) CD99 mouse monoclonal antibody, clone OTI5C1 (formerly 5C1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD99 (RC204056, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD99. Positive lysates LY400863 (100ug) and LC400863 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of A549 cell lysate (35ug) by using anti-CD99 monoclonal antibody. Dilution: 1:500

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD99 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800788)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD99 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800788)

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  Flow cytometric Analysis of living Jurkat cells, using anti-CD99 antibody (TA800788), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI5C1
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD99 (NP_002405) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 16.7 kDa
• Gene Name: Homo sapiens CD99 molecule (Xg blood group) (CD99), transcript variant 1, mRNA.
• Database Link:
  NP_002405
  Entrez Gene 4267 Human
  UniProt ID P14209
• Background: The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: HBA71; MIC2; MIC2X; MIC2Y; MSK5X

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Leukocyte transendothelial migration