Parathyroid Hormone (PTH) Mouse Monoclonal Antibody [Clone ID: OTI1B10]

CAT#: CF800912

Carrier-free (BSA/glycerol-free) PTH mouse monoclonal antibody, clone OTI1B10 (formerly 1B10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTH (RC219848, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTH. Positive lysates LY424804 (100ug) and LC424804 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1B10
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 32-115 of human PTH (NP_000306) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 9.4 kDa
• Gene Name: Homo sapiens parathyroid hormone (PTH), transcript variant 1, mRNA.
• Database Link:
  NP_000306
  Entrez Gene 5741 Human
  UniProt ID P01270
• Background: The protein encoded by this gene is a hormone secreted by parathyroid cells. This hormone elevates blood Ca2+ level by dissolving the salts in bone and preventing their renal excretion. Defects in this gene are a cause of familial isolated hypoparathyroidism (FIH). [provided by RefSeq, Jul 2008]
• Synonyms: PTH1

Reference Data

• Protein Families: Druggable Genome, Secreted Protein