CD99 Mouse Monoclonal Antibody [Clone ID: OTI1A1]

CAT#: CF800923

Carrier-free (BSA/glycerol-free) CD99 mouse monoclonal antibody,clone OTI1A1

Product Images

Western blot analysis of extracts (35ug) from Jurkat and HUVEC cell lines by using anti-KRT14 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CD99 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA800923) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-CD99 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA800923) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-CD99 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA (1:500)

Specifications

Product Data

• Clone Name: OTI1A1
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD99 (NP_002405) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 18.85 kDa
• Gene Name: Homo sapiens CD99 molecule (Xg blood group) (CD99), transcript variant 1, mRNA.
• Database Link:
  NP_002405
  Entrez Gene 4267 Human
  UniProt ID P14209
• Synonyms: HBA71; MIC2; MIC2X; MIC2Y; MSK5X

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Leukocyte transendothelial migration