EMA (MUC1) Mouse Monoclonal Antibody [Clone ID: OTI3A7]

CAT#: CF800938

Carrier-free (BSA/glycerol-free) MUC1 mouse monoclonal antibody, clone OTI3A7 (formerly 3A7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MUC1 (RC221390, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MUC1. Positive lysates LY422694 (100ug) and LC422694 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from T47D cells by using anti-MUC1 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MUC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800938)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MUC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800938)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MUC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800938)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MUC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800938)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MUC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800938)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MUC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800938)

• Immunofluorescent staining of MCF-7 cells using anti-MUC1 mouse monoclonal antibody (TA800938). Dilution: 1:100

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Specifications

Product Data

• Clone Name: OTI3A7
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MUC1 (NP_001018016) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25.1 kDa
• Gene Name: Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 2, mRNA.
• Database Link:
  NP_001018016
  Entrez Gene 4582 Human
  UniProt ID P15941
• Background: This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2011]
• Synonyms: ADMCKD; ADMCKD1; CA 15-3; CD227; EMA; H23AG; KL-6; MAM6; MCD; MCKD; MCKD1; MUC-1; SEC

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane