HE4 (WFDC2) Mouse Monoclonal Antibody [Clone ID: OTI1E12]

CAT#: CF801136

Carrier-free (BSA/glycerol-free) WFDC2 mouse monoclonal antibody, clone OTI1E12 (formerly 1E12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WFDC2 (RC208491, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WFDC2. Positive lysates LY416866 (100ug) and LC416866 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-WFDC2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801136)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-WFDC2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801136)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-WFDC2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801136)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-WFDC2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801136)

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Specifications

Product Data

• Clone Name: OTI1E12
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 31-124 of human WFDC2 (NP_006094) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 10 kDa
• Gene Name: Homo sapiens WAP four-disulfide core domain 2 (WFDC2), mRNA.
• Database Link:
  NP_006094
  Entrez Gene 10406 Human
  UniProt ID Q14508
• Background: This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. [provided by RefSeq, Jul 2008]
• Synonyms: dJ461P17.6; EDDM4; HE4; WAP5

Reference Data

• Protein Families: Secreted Protein, Transmembrane