p18 INK4c (CDKN2C) Mouse Monoclonal Antibody [Clone ID: OTI4G10]

CAT#: CF801229

Carrier-free (BSA/glycerol-free) CDKN2C mouse monoclonal antibody, clone OTI4G10 (formerly 4G10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2C (RC213083, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN2C. Positive lysates LY420041 (100ug) and LC420041 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI4G10
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CDKN2C (NP_001253) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 17.9 kDa
• Gene Name: Homo sapiens cyclin dependent kinase inhibitor 2C (CDKN2C), transcript variant 1, mRNA.
• Database Link:
  NP_001253
  Entrez Gene 12580 MouseEntrez Gene 54238 RatEntrez Gene 1031 Human
  UniProt ID P42773
• Background: The protein encoded by this gene is a member of the INK4 family of cyclin-dependent kinase inhibitors. This protein has been shown to interact with CDK4 or CDK6, and prevent the activation of the CDK kinases, thus function as a cell growth regulator that controls cell cycle G1 progression. Ectopic expression of this gene was shown to suppress the growth of human cells in a manner that appears to correlate with the presence of a wild-type RB1 function. Studies in the knockout mice suggested the roles of this gene in regulating spermatogenesis, as well as in suppressing tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode an identical protein, have been reported. [provided by RefSeq, Jul 2008]
• Synonyms: INK4C; p18; p18-INK4C

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle