MUC16 Mouse Monoclonal Antibody [Clone ID: OTI3B6]

CAT#: CF801281

Carrier-free (BSA/glycerol-free) MUC16 mouse monoclonal antibody, clone OTI3B6 (formerly 3B6)

Product Images

E.coli lysate (left lane) and E.coli lysate expressing Human recombinant protein fragment (right lane) corresponding to amino acids 10329-10628 of human MUC16 (NP_078966) were separated by SDS-PAGE and immunoblotted with anti-MUC16.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MUC16 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801281)

Specifications

Product Data

• Clone Name: OTI3B6
• Applications: IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 10329-10628 of human MUC16 (NP_078966) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens mucin 16, cell surface associated (MUC16), mRNA.
• Database Link:
  NP_078966
  Entrez Gene 94025 Human
  UniProt ID Q8WXI7
• Synonyms: CA125