RASGRP3 Mouse Monoclonal Antibody [Clone ID: OTI4G9]

CAT#: CF801446

Carrier-free (BSA/glycerol-free) RASGRP3 mouse monoclonal antibody, clone OTI4G9 (formerly 4G9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RASGRP3 (RC206487, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RASGRP3. Positive lysates LY403524 (100ug) and LC403524 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-RASGRP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801446)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RASGRP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801446)

Specifications

Product Data

• Clone Name: OTI4G9
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 380-690 of human RASGRP3 (NP_733772) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 78.2 kDa
• Gene Name: Homo sapiens RAS guanyl releasing protein 3 (RASGRP3), transcript variant 2, mRNA.
• Database Link:
  NP_733772
  Entrez Gene 25780 Human
  UniProt ID Q8IV61
• Background: Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RASGRP3, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]). [supplied by OMIM, Mar 2008]
• Synonyms: GRP3

Reference Data

• Protein Pathways: B cell receptor signaling pathway, MAPK signaling pathway