p95 NBS1 (NBN) Mouse Monoclonal Antibody [Clone ID: OTI6E11]

CAT#: CF801553

Carrier-free (BSA/glycerol-free) NBN mouse monoclonal antibody,clone OTI6E11

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NBN (RC214682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NBN. Positive lysates LY419300 (100ug) and LC419300 (20ug) can be purchased separately from OriGene.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and NBN-Knockout HeLa cells (KO, Cat# LC831313) were separated by SDS-PAGE and immunoblotted with anti-NBN monoclonal antibody TA801553 (1:500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801553)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801553)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801553)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801553)

• Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801553)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801553)

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801553)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801553)

• Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801553)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801553)

• Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801553)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801553)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801553)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801553)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801553)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801553)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801553)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801553)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801553)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801553)

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Specifications

Product Data

• Clone Name: OTI6E11
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 183-460 of human NBN (NP_002476)produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 84.8 kDa
• Gene Name: Homo sapiens nibrin (NBN), transcript variant 1, mRNA.
• Database Link:
  NP_002476
  Entrez Gene 4683 Human
  UniProt ID O60934
• Background: Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq, Jul 2008]
• Synonyms: AT-V1; AT-V2; ATV; NBS; NBS1; P95

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Homologous recombination