Ki67 (MKI67) Rat Monoclonal Antibody [Clone ID: OTI5D7]

CAT#: CF801577

Carrier-free (BSA/glycerol-free) MKI67 rat monoclonal antibody, clone OTI5D7 (formerly 5D7)

Product Images

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MKI67 rat monoclonal antibody. (TA801577; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MKI67 rat monoclonal antibody. (TA801577; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MKI67 rat monoclonal antibody. (TA801577; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MKI67 rat monoclonal antibody. (TA801577; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MKI67 rat monoclonal antibody. (TA801577; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MKI67 rat monoclonal antibody. (TA801577; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MKI67 rat monoclonal antibody. (TA801577; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MKI67 rat monoclonal antibody. (TA801577; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Flow cytometric analysis of fixed and permeabilized Jurkat cells, using anti-Ki67 antibody (TA801577 at 1:10, red), compare to IgG1 isotype control (Black).

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Specifications

Product Data

• Clone Name: OTI5D7
• Applications: FC, IHC
• Recommended Dilution: IHC 1:150
• Reactivities: Human
• Host: Rat
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1160-1493 of human MKI67 (NP_002408) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens marker of proliferation Ki-67 (MKI67), transcript variant 1, mRNA.
• Database Link:
  NP_002408
  Entrez Gene 4288 Human
  UniProt ID P46013
• Background: This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]
• Synonyms: KIA; MIB-; MIB-1; PPP1R105

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS