MelanA (MLANA) Mouse Monoclonal Antibody [Clone ID: OTI16D9]

CAT#: CF801608

Carrier-free (BSA/glycerol-free) MLANA mouse monoclonal antibody, clone OTI16D9 (formerly 16D9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MLANA (RC204190, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MLANA. Positive lysates LY417261 (100ug) and LC417261 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human embryonic liver tissue using anti-MLANA mouse monoclonal antibody. (TA801608)

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  Immunocytochemistry staining of SK-MEL-5 cells using anti-MLANA mouse monoclonal antibody (TA801608) (Left). The right is negative control (1:200).

Specifications

Product Data

• Clone Name: OTI16D9
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 48-118 of human MLANA (NP_005502) produced in E.coli
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 13 kDa
• Gene Name: Homo sapiens melan-A (MLANA), mRNA.
• Database Link:
  NP_005502
  Entrez Gene 2315 Human
  UniProt ID Q16655
• Synonyms: MART-1; MART1

Reference Data

• Protein Families: Transmembrane