CD23 (FCER2) Mouse Monoclonal Antibody [Clone ID: OTI1E5]

CAT#: CF801620

Carrier-free (BSA/glycerol-free) FCER2 mouse monoclonal antibody, clone OTI1E5 (formerly 1E5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCER2 (RC204335, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCER2 (1:2000).

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  Western blot analysis of extracts (35ug) from 2 cell lines lysates by using anti-FCER2 monoclonal antibody (1:500).

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  Flow cytometric analysis of living 293T cells transfected with FCER2 overexpression plasmid (RC204335), Red)/empty vector (PS100001, Blue) using anti-FCER2 antibody (TA801620). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

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  Flow cytometric analysis of living RPMI-8226 cells, using anti-FCER2 antibody (TA801620, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).

Specifications

Product Data

• Clone Name: OTI1E5
• Applications: FC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 48-321 of human FCER2 (NP_001993) produced in SF9 cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36.3 kDa
• Gene Name: Homo sapiens Fc fragment of IgE receptor II (FCER2), transcript variant 1, mRNA.
• Database Link:
  NP_001993
  Entrez Gene 2208 Human
  UniProt ID P06734
• Background: The protein encoded by this gene is a B-cell specific antigen, and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation, and the regulation of IgE production. This protein also exists as a soluble secreted form, then functioning as a potent mitogenic growth factor. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul
• Synonyms: BLAST-2; CD23; CD23A; CLEC4J; FCE2; IGEBF

Reference Data

• Protein Families: Secreted Protein, Transmembrane
• Protein Pathways: Hematopoietic cell lineage