Dicer (DICER1) Mouse Monoclonal Antibody [Clone ID: OTI1G8]

CAT#: CF801644

Carrier-free (glycerol/BSA-free) DICER1 mouse monoclonal antibody, clone OTI1G8 (formerly 1G8)

Product Images

Western blot analysis of HT29 cell lysate (35ug) by using anti-DICER1 monoclonal antibody. Dilution: 1:500

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DICER1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801644)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DICER1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801644)

Specifications

Product Data

• Clone Name: OTI1G8
• Applications: IHC, WB
• Recommended Dilution: IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1666-1922 of human DICER1 (NP_085124) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 218.5 kDa
• Gene Name: Homo sapiens dicer 1, ribonuclease III (DICER1), transcript variant 2, mRNA.
• Database Link:
  NP_085124
  Entrez Gene 192119 MouseEntrez Gene 299284 RatEntrez Gene 23405 Human
  UniProt ID Q9UPY3
• Background: This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep
• Synonyms: DCR1; Dicer; Dicer1e; HERNA; K12H4.8-LIKE; MNG1; RMSE2

Reference Data

• Protein Families: Druggable Genome