CD30 (TNFRSF8) Mouse Monoclonal Antibody [Clone ID: OTI18D3]

CAT#: CF801647

Carrier-free (BSA/glycerol-free) TNFRSF8 mouse monoclonal antibody, clone OTI18D3 (formerly 18D3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF8 (RC219819, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNFRSF8. Positive lysates LY420050 (100ug) and LC420050 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-TNFRSF8 mouse monoclonal antibody. (TA801647)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TNFRSF8 mouse monoclonal antibody. (TA801647)

• 

  Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-TNFRSF8 mouse monoclonal antibody. (TA801647)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TNFRSF8 mouse monoclonal antibody. (TA801647)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TNFRSF8 mouse monoclonal antibody. (TA801647)

• Flow cytometric Analysis of K562 and Ramos cells, using anti-TNFRSF8 antibody (TA801647), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue) (1:100).

• Flow cytometric Analysis of K562 cells, using anti-TNFRSF8 antibody (TA801647), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue). Dilution: 1:50

• Flow cytometric analysis of TNFRSF8 expression on stimulated human peripheral blood mononuclear cells (PBMC).Unstimulated PBMC (Left) or 10ug/ml Phytohemagglutinin and 100U/ml IL-2-stimulated (3 days) PBMC (Right) were stained with Anti-Human TNFRSF8 antibody (TA801647) (1:100).

Show all 9 images

Specifications

Product Data

• Clone Name: OTI18D3
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 19-379 of human TNFRSF8 (NP_001234) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 61.9 kDa
• Gene Name: Homo sapiens TNF receptor superfamily member 8 (TNFRSF8), transcript variant 1, mRNA.
• Database Link:
  NP_001234
  Entrez Gene 943 Human
  UniProt ID P28908
• Background: The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
• Synonyms: CD30; D1S166E; Ki-1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction