PIK3CD Mouse Monoclonal Antibody [Clone ID: OTI2H3]

CAT#: CF801891

Carrier-free (glycerol/BSA-free) PIK3CD mouse monoclonal antibody, clone OTI2H3 (formerly 2H3)

Product Images

E.coli lysate (left lane) and E.coli lysate expressing Human recombinant protein fragment (right lane) corresponding to amino acids 286-610 of human PIK3CD (NP_005017).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801891)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801891)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801891)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801891)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801891)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801891)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801891)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801891)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PIK3CD mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801891)

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Specifications

Product Data

• Clone Name: OTI2H3
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 286-610 of human PIK3CD (NP_005017) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 119.3 kDa
• Gene Name: Homo sapiens phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta (PIK3CD), transcript variant 1, mRNA.
• Database Link:
  NP_005017
  Entrez Gene 18707 MouseEntrez Gene 366508 RatEntrez Gene 5293 Human
  UniProt ID O00329
• Background: Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by this gene is a class I PI3K found primarily in leukocytes. Like other class I PI3Ks (p110-alpha p110-beta, and p110-gamma), the encoded protein binds p85 adapter proteins and GTP-bound RAS. However, unlike the other class I PI3Ks, this protein phosphorylates itself, not p85 protein. [provided by RefSeq, Jul
• Synonyms: APDS; IMD14; p110D; P110DELTA; PI3K

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Acute myeloid leukemia, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Glioma, Inositol phosphate metabolism, Insulin signaling pathway, Jak-STAT signaling pathway, Leukocyte transendothelial migration, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Phosphatidylinositol signaling system, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus, VEGF signaling pathway