CD5 Mouse Monoclonal Antibody [Clone ID: OTI1A5]

CAT#: CF801941

Carrier-free (BSA/glycerol-free) CD5 mouse monoclonal antibody, clone OTI1A5 (formerly 1A5)

Product Images

Living HEK293T cells transfected with either RC206494 overexpress plasmid (Red), compared to an IgG isotype control, (Green) or empty vector control plasmid (Blue) were immunostained by anti-CD5 antibody (TA801941), and then analyzed by flow cytometry (1:100).

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  Flow cytometric analysis of living 50ng/ml IL2-stimulated Jurkat cells, using anti-CD5 antibody (TA801941), (red), compared to an IgG isotype control, (green), and negative control (PBS), (blue) (1:100).

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  Flow cytometric analysis of living CCRF-CEM cells, using anti-CD5 antibody (TA801941), (red), compared to an IgG isotype control, (green), and negative control (PBS), (blue) (1:100).

Specifications

Product Data

• Clone Name: OTI1A5
• Applications: FC
• Recommended Dilution: FLOW 1:50
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD5 (NP_055022) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.4 kDa
• Gene Name: Homo sapiens CD5 molecule (CD5), transcript variant 1, mRNA.
• Database Link:
  NP_055022
  Entrez Gene 921 Human
  UniProt ID P06127
• Synonyms: LEU1; T1

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Hematopoietic cell lineage