Hamartin (TSC1) Mouse Monoclonal Antibody [Clone ID: OTI2B2]

CAT#: CF802012

Carrier-free (BSA/glycerol-free) TSC1 mouse monoclonal antibody, clone OTI2B2 (formerly 2B2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC1 (RC213332, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC1. Positive lysates LY400132 (100ug) and LC400132 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2B2
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 836-1164 of human TSC1 (NP_000359) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 129.6 kDa
• Gene Name: Homo sapiens TSC complex subunit 1 (TSC1), transcript variant 1, mRNA.
• Database Link:
  NP_000359
  Entrez Gene 60445 RatEntrez Gene 64930 MouseEntrez Gene 7248 Human
  UniProt ID Q92574
• Background: This gene encodes a growth inhibitory protein thought to play a role in the stabilization of tuberin. Mutations in this gene have been associated with tuberous sclerosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun
• Synonyms: LAM; TSC

Reference Data

• Protein Pathways: Insulin signaling pathway, mTOR signaling pathway