Kallikrein 2 (KLK2) Mouse Monoclonal Antibody [Clone ID: OTI5D6]

CAT#: CF802077

Carrier-free (BSA/glycerol-free) KLK2 mouse monoclonal antibody, clone OTI5D6 (formerly 5D6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY KLK2 (Cat# RC202667, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KLK2(Cat# TA802077). Positive lysates LY417229 (100ug) and LC417229 (20ug) can be purchased separately from OriGene.

• 

  Western blot analyses for the cross-reactivity between KLK2 and KLK3 overexpression lysate. The results indicate that KLK3 overexpressed lysate is not getting detected by the anti-KLK2 antibody (TA802077)

• 

  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-KLK2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Specifications

Product Data

• Clone Name: OTI5D6
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 25-261 of human KLK2 (NP_005542) produced in SF9 cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28.5 kDa
• Gene Name: kallikrein related peptidase 2
• Database Link:
  NP_005542
  Entrez Gene 3817 Human
  UniProt ID P20151
• Background: This gene encodes a member of the grandular kallikrein protein family. Kallikreins are a subgroup of serine proteases that are clustered on chromosome 19. Members of this family are involved in a diverse array of biological functions. The protein encoded by this gene is a highly active trypsin-like serine protease that selectively cleaves at arginine residues. This protein is primarily expressed in prostatic tissue and is responsible for cleaving pro-prostate-specific antigen into its enzymatically active form. This gene is highly expressed in prostate tumor cells and may be a prognostic maker for prostate cancer risk. Alternate splicing results in both coding and non-coding transcript variants. [provided by RefSeq, Jan 2012]
• Synonyms: hGK-1; hK2; KLK2A2

Reference Data

• Protein Families: Druggable Genome, Protease