NUMB Mouse Monoclonal Antibody [Clone ID: OTI3E5]

CAT#: CF802169

Carrier-free (BSA/glycerol-free) NUMB mouse monoclonal antibody, clone OTI3E5 (formerly 3E5)

Formulation: Standard Carrier Free


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USD 465.00

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Size
    • 100 ug

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Specifications

Product Data
Clone Name OTI3E5
Applications IHC, WB
Recommended Dilution WB 1:2000, IHC 1:150
Reactivities Human, Mouse, Rat
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Human recombinant protein fragment corresponding to amino acids 1-365 of human NUMB (NP_001005744) produced in E.coli.
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 65.7 kDa
Gene Name Homo sapiens NUMB endocytic adaptor protein (NUMB), transcript variant 2, mRNA.
Background The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Synonyms C14orf41; c14_5527; S171
Reference Data
Protein Pathways Notch signaling pathway

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