DDIT3 Mouse Monoclonal Antibody [Clone ID: OTI3H7]

CAT#: CF802210

Carrier-free (BSA/glycerol-free) DDIT3 mouse monoclonal antibody,clone OTI3H7

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDIT3 (RC201301, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDIT3. Positive lysates LY418226 (100ug) and LC418226 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-DDIT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA802210) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-DDIT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA802210) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DDIT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA802210) (1:500)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-DDIT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA802210) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DDIT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA802210) (1:500)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-DDIT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA802210) (1:500)

• Immunohistochemical staining of paraffin-embedded Human Gastric Carcinoma using anti-DDIT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA802210) (1:500)

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Specifications

Product Data

• Clone Name: OTI3H7
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DDIT3 (NP_004074) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 19 kDa
• Gene Name: Homo sapiens DNA damage inducible transcript 3 (DDIT3), transcript variant 5, mRNA.
• Database Link:
  NP_004074
  Entrez Gene 13198 MouseEntrez Gene 29467 RatEntrez Gene 1649 Human
  UniProt ID P35638
• Background: This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010]
• Synonyms: CEBPZ; CHOP; CHOP-10; CHOP10; GADD153

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: MAPK signaling pathway