AREB6 (ZEB1) Mouse Monoclonal Antibody [Clone ID: OTI8E2]

CAT#: CF802295

Carrier-free (BSA/glycerol-free) ZEB1 mouse monoclonal antibody, clone OTI8E2 (formerly 8E2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZEB1 (RC217704, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZEB1. Positive lysates LY403073 (100ug) and LC403073 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 1 cell line by using anti-ZEB1 monoclonal antibody at 1:200.

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ZEB1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Specifications

Product Data

• Clone Name: OTI8E2
• Applications: IHC, WB
• Recommended Dilution: WB 1:200 - 1:1000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-350 of human ZEB1 (NP_110378) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: zinc finger E-box binding homeobox 1
• Database Link:
  NP_110378
  Entrez Gene 21417 MouseEntrez Gene 25705 RatEntrez Gene 6935 Human
  UniProt ID P37275
• Background: This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2010]
• Synonyms: AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3; TCF8; ZFHEP; ZFHX1A

Reference Data

• Protein Families: Transcription Factors