FBXO11 Mouse Monoclonal Antibody [Clone ID: OTI1F6]

CAT#: CF802323

Carrier-free (BSA/glycerol-free) FBXO11 mouse monoclonal antibody,clone OTI1F6

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FBXO11 (RC222041, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FBXO11. Positive lysates LY403052 (100ug) and LC403052 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of HT29 cell lysate (35ug) by using anti-FBXO11 monoclonal antibody. Dilution: 1:500

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and FBXO11-Knockout HeLa cells (KO, Cat# LC810452) were separated by SDS-PAGE and immunoblotted with anti-FBXO11 monoclonal antibody TA802323 (1:100`). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

Specifications

Product Data

• Clone Name: OTI1F6
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-300 of human FBXO11 (NP_079409) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 93.9 kDa
• Gene Name: Homo sapiens F-box protein 11 (FBXO11), transcript variant 1, mRNA.
• Database Link:
  NP_079409
  Entrez Gene 80204 Human
  UniProt ID Q86XK2
• Background: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. It can function as an arginine methyltransferase that symmetrically dimethylates arginine residues, and it acts as an adaptor protein to mediate the neddylation of p53, which leads to the suppression of p53 function. This gene is known to be down-regulated in melanocytes from patients with vitiligo, a skin disorder that results in depigmentation. Polymorphisms in this gene are associated with chronic otitis media with effusion and recurrent otitis media (COME/ROM), a hearing loss disorder, and the knockout of the homologous mouse gene results in the deaf mouse mutant Jeff (Jf), a single gene model of otitis media. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jun 2010]
• Synonyms: FBX11; PRMT9; UBR6; UG063H01; VIT1

Reference Data

• Protein Families: Druggable Genome