NDUFS2 Mouse Monoclonal Antibody [Clone ID: OTI2G2]

CAT#: CF802325

Carrier-free (BSA/glycerol-free) NDUFS2 mouse monoclonal antibody, clone OTI2G2 (formerly 2G2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDUFS2 (RC203485, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDUFS2. Positive lysates LY417919 (100ug) and LC417919 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of A549 cell lysate (35ug) by using anti-NDUFS2 monoclonal antibody. Dilution: 1:500

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-NDUFS2 mouse monoclonal antibody. (TA802325) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NDUFS2 mouse monoclonal antibody. (TA802325) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NDUFS2 mouse monoclonal antibody. (TA802325) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NDUFS2 mouse monoclonal antibody. (TA802325) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NDUFS2 mouse monoclonal antibody. (TA802325) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NDUFS2 mouse monoclonal antibody. (TA802325) Dilution: 1:150

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Specifications

Product Data

• Clone Name: OTI2G2
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 205-463 of human NDUFS2 (NP_004541) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49.1 kDa
• Gene Name: Homo sapiens NADH:ubiquinone oxidoreductase core subunit S2 (NDUFS2), transcript variant 1, mRNA.
• Database Link:
  NP_004541
  Entrez Gene 226646 MouseEntrez Gene 289218 RatEntrez Gene 4720 Human
  UniProt ID O75306
• Background: The protein encoded by this gene is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Mammalian mitochondrial complex I is composed of at least 43 different subunits, 7 of which are encoded by the mitochondrial genome, and the rest are the products of nuclear genes. The iron-sulfur protein fraction of complex I is made up of 7 subunits, including this gene product. Complex I catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mutations in this gene are associated with mitochondrial complex I deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
• Synonyms: CI-49

Reference Data

• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease