SOX9 Mouse Monoclonal Antibody [Clone ID: OTI2H10]

CAT#: CF802387

Carrier-free (BSA/glycerol-free) SOX9 mouse monoclonal antibody, clone OTI2H10 (formerly 2H10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOX9 (RC208944, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOX9. Positive lysates LY424779 (100ug) and LC424779 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of HT29 cell lysate (35ug) by using anti-SOX9 monoclonal antibody. Dilution: 1:500

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SOX9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, TA802387)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SOX9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, TA802387)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SOX9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, TA802387)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SOX9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, TA802387)

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Specifications

Product Data

• Clone Name: OTI2H10
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-205 of human SOX9 (NP_000337) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 56 kDa
• Gene Name: Homo sapiens SRY-box transcription factor 9 (SOX9), mRNA.
• Database Link:
  NP_000337
  Entrez Gene 20682 MouseEntrez Gene 140586 RatEntrez Gene 6662 Human
  UniProt ID P48436
• Background: The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. [provided by RefSeq, Jul 2008]
• Synonyms: CMD1; CMPD1; SRA1; SRXX2; SRXY10

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors