Ornithine Carbamoyltransferase (OTC) Mouse Monoclonal Antibody [Clone ID: OTI8A1]

CAT#: CF802394

Carrier-free (BSA/glycerol-free) OTC mouse monoclonal antibody, clone OTI8A1 (formerly 8A1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OTC (RC214662, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OTC.

• 

  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-OTC mouse monoclonal antibody. (TA802394) Dilution: 1:150

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-OTC mouse monoclonal antibody. (TA802394) Dilution: 1:150

Specifications

Product Data

• Clone Name: OTI8A1
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 33-354 of human OTC (NP_000522)produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36.1 kDa
• Gene Name: Homo sapiens ornithine carbamoyltransferase (OTC), mRNA; nuclear gene for mitochondrial product.
• Database Link:
  NP_000522
  Entrez Gene 18416 MouseEntrez Gene 25611 RatEntrez Gene 5009 Human
  UniProt ID P00480
• Background: This nuclear gene encodes a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia. Since the gene for this enzyme maps close to that for Duchenne muscular dystrophy, it may play a role in that disease also. [provided by RefSeq, Jul 2008]
• Synonyms: OCTD

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways