CD5 Mouse Monoclonal Antibody [Clone ID: OTI1A8]

CAT#: CF802403

Carrier-free (BSA/glycerol-free) CD5 mouse monoclonal antibody, clone OTI1A8 (formerly 1A8)

Product Images

HEK293T cells transfected with either RC206494 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CD5 antibody (TA802403), and then living cells were analyzed by flow cytometry.

• 

  Flow cytometric analysis of living 50ng/ml IL2-stimulated Jurkat cells, using anti-CD5 antibody (TA802403), (red), compared to an IgG isotype control, (green), and negative control (PBS), (blue) (1:100).

• 

  Flow cytometric analysis of living CCRF-CEM cells, using anti-CD5 antibody (TA802403), (red), compared to an IgG isotype control, (green), and negative control (PBS), (blue) (1:100).

Specifications

Product Data

• Clone Name: OTI1A8
• Applications: FC
• Recommended Dilution: FLOW 1:50
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD5 (NP_055022) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.4 kDa
• Gene Name: Homo sapiens CD5 molecule (CD5), transcript variant 1, mRNA.
• Database Link:
  NP_055022
  Entrez Gene 921 Human
  UniProt ID P06127
• Synonyms: LEU1; T1

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Hematopoietic cell lineage