GAPDH Mouse Monoclonal Antibody [Clone ID: OTI2D9]

CAT#: CF802519

Carrier-free (BSA/glycerol-free) GAPDH mouse monoclonal antibody, clone OTI2D9 (formerly 2D9)

Product Images

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GAPDH monoclonal antibody.

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  Figure from citation: Western Blot of GAPDH protein level by using anti-GAPDH antibody in human renal cells. View Citation

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  Figure from citation: Western Blot of GAPDH protein level by using anti-GAPDH antibody in human pancreatic adenocarcinoma cell lines. Dilution: 1:2500 View Citation

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  Figure from citation: Western Blot of GAPDH protein level by using anti-GAPDH antibody in human K562 cells. Dilution: 1:1000 View Citation

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  Figure from citation: Western Blot of GAPDH protein level by using anti-GAPDH antibody in HEK293 cells. Dilution: 1:500 View Citation

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  Figure from citation: Western blot analysis of GAPDH protein level by using anti-GAPDH antibody in SAOS-2 cells. Dilution: 1:2000 View Citation

• Figure from citation: Western blot analysis of GAPDH protein level by using anti-GAPDH antibody (TA802519) in various cell lines, GAPDH used as loading control. View Citation

• Figure from citation: Western blot analysis of GAPDH protein level by using anti-GAPDH antibody in fibroblasts, GAPDH used as loading control. View Citation

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Specifications

Product Data

• Clone Name: OTI2D9
• Applications: WB
• Recommended Dilution: WB 1:1000
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GAPDH (NP_002037) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35.9 kDa
• Gene Name: Homo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH), transcript variant 1, mRNA.
• Database Link:
  NP_002037
  Entrez Gene 14433 MouseEntrez Gene 24383 RatEntrez Gene 403755 DogEntrez Gene 574353 MonkeyEntrez Gene 2597 Human
  UniProt ID P04406
• Background: The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]
• Synonyms: G3PD; GAPD; HEL-S-162eP

Reference Data

• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways