PARN Mouse Monoclonal Antibody [Clone ID: OTI1G5]

CAT#: CF802649

Carrier-free (BSA/glycerol-free) PARN mouse monoclonal antibody,clone OTI1G5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PARN (RC207220, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PARN. Positive lysates LY419233 (100ug) and LC419233 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of HT29 cell lysate (35ug) by using anti-PARN monoclonal antibody. Dilution: 1:500

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PARN mouse monoclonal antibody. (TA802649) Dilution: 1:150

• 

  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PARN mouse monoclonal antibody. (TA802649) Dilution: 1:150

• 

  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PARN mouse monoclonal antibody. (TA802649) Dilution: 1:150

• 

  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PARN mouse monoclonal antibody. (TA802649) Dilution: 1:150

Show all 6 images

Specifications

Product Data

• Clone Name: OTI1G5
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-266 of human PARN (NP_002573) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens poly(A)-specific ribonuclease (PARN), transcript variant 1, mRNA.
• Database Link:
  NP_002573
  Entrez Gene 74108 MouseEntrez Gene 5073 Human
  UniProt ID O95453
• Synonyms: DAN

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: RNA degradation