DOCK2 Mouse Monoclonal Antibody [Clone ID: OTI7G2]

CAT#: CF802698

Carrier-free (BSA/glycerol-free) DOCK2 mouse monoclonal antibody, clone OTI7G2 (formerly 7G2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOCK2 (RC211198, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOCK2.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-DOCK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802698)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-DOCK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802698)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-DOCK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802698)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-DOCK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802698)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-DOCK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802698)

• Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-DOCK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802698)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DOCK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802698)

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Specifications

Product Data

• Clone Name: OTI7G2
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1544-1830 of human DOCK2 (NP_004937) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens dedicator of cytokinesis 2 (DOCK2), transcript variant 1, mRNA.
• Database Link:
  NP_004937
  Entrez Gene 94176 MouseEntrez Gene 360509 RatEntrez Gene 1794 Human
  UniProt ID Q92608
• Background: The protein encoded by this gene belongs to the CDM protein family. It is specifically expressed in hematopoietic cells, predominantly in the peripheral blood leukocytes, and is involved in remodeling of the actin cytoskeleton required for lymphocyte migration, through the activation of RAC. Mice lacking this gene show a severe impairment in the migration and homing of lymphocytes. These mutant mice also exhibited long-term survival of allografts, suggesting that this gene may be a target for controlling transplant rejection. [provided by RefSeq, Oct 2011]
• Synonyms: FLJ46592; KIAA0209

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Chemokine signaling pathway, Fc gamma R-mediated phagocytosis