DOCK2 Mouse Monoclonal Antibody [Clone ID: OTI4E12]

CAT#: CF802699

Carrier-free (glycerol/BSA-free) DOCK2 mouse monoclonal antibody, clone OTI4E12 (formerly 4E12)

Product Images

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-DOCK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA802699)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DOCK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA802699)

• 

  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-DOCK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA802699)

Specifications

Product Data

• Clone Name: OTI4E12
• Applications: IHC
• Recommended Dilution: IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1544-1830 of human DOCK2 (NP_004937) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens dedicator of cytokinesis 2 (DOCK2), transcript variant 1, mRNA.
• Database Link:
  NP_004937
  Entrez Gene 94176 MouseEntrez Gene 360509 RatEntrez Gene 1794 Human
  UniProt ID Q92608
• Synonyms: FLJ46592; KIAA0209

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Chemokine signaling pathway, Fc gamma R-mediated phagocytosis