PARN Mouse Monoclonal Antibody [Clone ID: OTI2D9]

CAT#: CF802721

Carrier-free (BSA/glycerol-free) PARN mouse monoclonal antibody,clone OTI2D9

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PARN (RC207220, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PARN. Positive lysates LY419233 (100ug) and LC419233 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PARN mouse monoclonal antibody. (TA802721) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PARN mouse monoclonal antibody. (TA802721) Dilution: 1:150

Specifications

Product Data

• Clone Name: OTI2D9
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-266 of human PARN (NP_002573) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 73.3 kDa
• Gene Name: Homo sapiens poly(A)-specific ribonuclease (PARN), transcript variant 1, mRNA.
• Database Link:
  NP_002573
  Entrez Gene 74108 MouseEntrez Gene 5073 Human
  UniProt ID O95453
• Background: The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
• Synonyms: DAN

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: RNA degradation